Structural analysis of glycoprotein sialylation – Part I: pre-LC-MS analytical strategies

Analytical strategies for LC-MS-based targeted metabolomics.

Recent advances in mass spectrometry are enabling improved analysis of endogenous metabolites. Here we discuss several issues relevant to developing liquid chromatography-electrospray ionization-mass spectrometry methods for targeted metabolomics (i.e., quantitative analysis of dozens to hundreds of specific metabolites). Sample preparation and liquid chromatography approaches are discussed, wi...

Analytical Considerations When Monitoring Pain Medications by LC-MS/MS

Urine drug testing of patients on chronic opioid therapy is a recognized component of the standard of care for this population [1-3]. Because a significant portion of this population experiences symptoms of other conditions including anxiety and depression, treatment often involves multiple medications. Additionally, a small percentage of these people take non-prescribed medications and/or illi...

Global analysis of modifications of the human BK virus structural proteins by LC-MS/MS.

BK virus, a human polyomavirus, may cause nephritis and urological disorders in patients who have undergone renal transplantation. Little is known about the characteristics of the BK viral proteins. In the current study, BK viral proteins were characterized by immunoblotting and LC-MS/MS. The results revealed that BK virus is composed of three structural proteins, VP1, VP2, and VP3 and four cel...

Part I: Analytical Considerations

IM A G E : LA G U N A D E S IG N /G E TT Y IM A G E S The United States Pharmacopeial Convention (USP) Therapeutic Peptides Expert Panel was formed in 2013 to evaluate quality attributes for synthetic peptides based on currently available regulatory guidance and expectations. Public quality standards for drug products and drug substances are developed by USP and enforceable by FDA. This series ...

Metabolomics: Lc-ms Analysis Development